Journal: Journal of Inflammation Research

Article Title: Electroacupuncture Pretreatment at Zusanli (ST36) Ameliorates Hepatic Ischemia/Reperfusion Injury in Mice by Reducing Oxidative Stress via Activating Vagus Nerve-Dependent Nrf2 Pathway

doi: 10.2147/JIR.S404087

Figure Lengend Snippet: EA activated the Nrf2 pathway in mice hepatic IRI. ( A and B ) Serum MDA and SOD levels. ( C and D ) The mRNA and protein levels of Nrf2. ( E – G ) HO-1, NQO1 expression in the liver tissues. Lamin B and β-actin were used as loading controls. All data were presented as the mean ± SD. * P <0.05, ** P < 0.01, *** P < 0.001 (n=3–5 per group).

Article Snippet: The primary antibodies against F4/80, MPO, P-NF-κb, NF-κb, P-IκBα, IκBα, Nrf2 (Cell signaling technology, 70076, 14569, 3033, 8242, 2859, 4812, 12721), β-Actin (ABGENT, AP14779b), HO-1, NQO-1, Lamin B2 (Abmart, T55113, T56710, T57109) were used.

Techniques: Expressing

Journal: Journal of Inflammation Research

Article Title: Electroacupuncture Pretreatment at Zusanli (ST36) Ameliorates Hepatic Ischemia/Reperfusion Injury in Mice by Reducing Oxidative Stress via Activating Vagus Nerve-Dependent Nrf2 Pathway

doi: 10.2147/JIR.S404087

Figure Lengend Snippet: ML385 reversed the protective impact of EA on liver IRI. ( A ) The experimental design. ( B ) H&E staining images of liver tissues (original magnification, × 100) showed necrotic areas. ( C and D ) ALT and AST levels in serum. ( E and F ) Serum levels of indicated cytokines. ( G ) Nuclear Nrf2, HO-1 and NQO1 expression in the liver tissues. Lamin B and β-actin were used as loading controls. All data were presented as the mean ± SD. * P <0.05, ** P < 0.01, *** P < 0.001 (n=3–5 per group).

Article Snippet: The primary antibodies against F4/80, MPO, P-NF-κb, NF-κb, P-IκBα, IκBα, Nrf2 (Cell signaling technology, 70076, 14569, 3033, 8242, 2859, 4812, 12721), β-Actin (ABGENT, AP14779b), HO-1, NQO-1, Lamin B2 (Abmart, T55113, T56710, T57109) were used.

Techniques: Staining, Expressing

Journal: Journal of Inflammation Research

Article Title: Electroacupuncture Pretreatment at Zusanli (ST36) Ameliorates Hepatic Ischemia/Reperfusion Injury in Mice by Reducing Oxidative Stress via Activating Vagus Nerve-Dependent Nrf2 Pathway

doi: 10.2147/JIR.S404087

Figure Lengend Snippet: Vagotomy eliminated the protective action of EA on liver IRI and inhibits the Nrf2 pathway. ( A ) Display of the left vagus nerve and the experimental design. ( B ) H&E staining images of liver tissues (original magnification, × 100) showed necrotic areas. ( C and D ) Serum levels of ALT and AST. ( E and F ) Serum levels of indicated cytokines. ( G ) Nuclear Nrf2, HO-1 and NQO1 expression in the liver tissues. Lamin B and β-actin were used as loading controls. All data were presented as the mean ± SD. * P <0.05, ** P < 0.01, *** P < 0.001 (n=3–5 per group).

Article Snippet: The primary antibodies against F4/80, MPO, P-NF-κb, NF-κb, P-IκBα, IκBα, Nrf2 (Cell signaling technology, 70076, 14569, 3033, 8242, 2859, 4812, 12721), β-Actin (ABGENT, AP14779b), HO-1, NQO-1, Lamin B2 (Abmart, T55113, T56710, T57109) were used.

Techniques: Staining, Expressing

Journal: The American Journal of Pathology

Article Title: Coronavirus Disease 2019 (COVID-19) Coronary Vascular Thrombosis

doi: 10.1016/j.ajpath.2021.09.004

Figure Lengend Snippet: Neutrophils contribute to thrombosis in patients. A: The number of neutrophil-platelet aggregates per 20× field was quantified in patients and controls. B and C: Patient tissue images of neutrophil-platelet aggregates and a neutrophil during NETosis, capturing platelets. D: Neutrophil rich macrothrombi composed of more than 30% neutrophils were counted in entire tissue at 10×. Values were normalized by tissue area. E and F: Images showing neutrophil macrothrombi and neutrophil aggregates that are CitH3+ in circulation in COVID-19 heart tissue. G: Image shows neutrophils with increased p65 expression ( arrows ) in COVID-19 tissue. Myeloperoxidase (MPO) was used to identify neutrophils and Citrullinated histone H3 (CitH3) to identify NET formation. Data represent means ± SEM. ∗ P < 0.05. Scale bars: 50 μm ( B , C , E , and F ); 100 μm ( G ).

Article Snippet: Primary antibodies reactive with the following antigens were added overnight at 4°C: Cleaved Caspase 3 (1:300, Cell Signaling Technology (CST) 5A1E), ICAM-1 (1:300, Novus Biologicals (NB) BBA17), myeloperoxidase (1:200, CST E1E7I), CD45 (1:100 Abcam (ab) ab10558), CD42b (1:100, Thermo Fisher (TF) 42C01; 1:150, TF PA5-109282), VE-Cadherin (1:300, SantaCruz (sc) sc-6458), Fibrin (1:200, Dako A0080), IgM AF647 (1:200 SouthernBiotech (SB) 2020-31), CD11b (1:100 TF M1/70), myeloperoxidase AF647 (1:100, ab252131), CitH3 (1:100, ab5103), p65 (1:400, CST D14E12), pSTAT1 (1:100, CST 58D6), pSTAT3 (1:100, CST D3A7), tissue factor (1:200, CST E9M6T; 1:200 NBP2-61641), VWF (1:50, ab201336), VCAM-1 (1:100, NB 6G9), E-selectin (1:100, TF PA5-96091), SARS CoV-2 spike (1:200, GeneTex GTX632604), SARS CoV-2 nucleocapsid (1:200, CST HL344), and C4d (1:100, Quidel A213).

Techniques: Expressing